Isoelectric focusing techniques and devices

ABSTRACT

Ampholytes are rapidly separated with high resolution in preparative scale by isoelectric focusing at high power inputs. An improved technique for isoelectric focusing involves sequential treatment of an ampholyte mixture in successively smaller focusing units without substantial dilution. Improved isoelectric focusing devices operating at high power inputs, with highly efficient heat dissipation, are described.

[451 Aug. 26, 1975 United States Patent n91 Denckla [54] [SOELECTRIC FOCUSING TECHNIQUES 3.616.456 l0/l97l 204/299 AND DEVICES 03 lI/l973 Grubhofer et al. 204/[80 R [75] Inventor: William Donner Denckla, Tenafly,

Primary Examiner-John H. Mack Assistant Examiner--A. C. Prescott [73] Assignee: Hoffmann-La Roche Inc., Nutley, Attorney, ge Firmsamuel Welt; 5-

NJ. Saxe; George M. Gould [57] ABSTRACT Ampholytes are rapidly separated with high resolution [22] Filed: July 10, 1974 Appl. No: 487,081

in preparative scale by isoelectric focusing at high power inputs. An improved technique for isoelectric focusing involves sequential treatment of an ampho- [52] U.S. 204/180 R; 204/299 [51] Int. Cl. ...i.v.. 801K 5/00 204/l80 G, I80 R, 299

[53] Field f e mixture in successively smaller focusing units without substantial dilution. Improved isoelectric focusing devices operating at high power inputs, with highly efficient heat dissipation lyt References Cited UNITED STATES PATENTS are described.

3,497,438 Badgley....4..................... 204/[80 R 3 Claims, 3 Drawing Figures n M QQQQQQQQ it lu F y a m s H w x H M III I 10711 w m 5; s x M 3 w m .(2 s M s 1 k \\\\\\\\\\\\K\\\\v m\\\\\\\\\\ 3 ISOELECTRIC FOCUSING TECHNIQUES AND DEVICES BACKGROUND OF THE INVENTION The separation and purification of complex molecules of biological interest has been the subject of extensive research. Since many of these molecules are ampholytes, they are theoretically amenable to separation by electrophoretic techniques. A mixture of ampholytes in solution, when subjected to a direct current electric potential will arrange within this electric potential according to isoelectric point, thereby resulting in separation.

In recent years, an improved technique for separation of ampholytes, known as isoelectric focusing, has been developed. According to this technique, an artificial pH gradient is established by use of a mixture of synthetic compounds (referred to as carrier ampholytes). Upon electrophoresis of an ampholyte mixture in such a pH gradient, which acts as buffer, the ampholytes arrange themselves within this gradient. In essence, each ampholyte molecule migrates towards the pH value in the gradient where it is isoelectric, that is, where its net charge is 0. The focusing thus takes place at the point where the pH is equal to the pl of the ampholyte.

Detailed treatment of the theory and techniques of isoelectric focusing may be found in Haglund, Methods of Biochemical Analysis, Vol. 19, pg 1; and Annals of the New York Academy of Sciences, Vol. 209, lsoelectric Focusing and lsotachophoresis"v Recently, a series of devices for use in isoelectric focasing have been described in Valmet in US. Pat. No. 3,616,456 and Science Tools, Vol. 16, pg 1 (1969). These devices have a series of chambers (usually U- tubes or V-tubes" disposed one after the other. The advantage of such a system is that if contamination occurs in one chamber, for example by precipitation ofa solid, this does not result in contamination of the entire system. These devices were described as operating at relatively low voltages, from about 800 to 2400 volts, and with a power input of about to watts for a 45 ml unit. Under these conditions, there is a limitation upon the resolution, the capacity, and, particularly, the speed of an electrophoretic separation since speed is directly proportional to the applied voltage and resolution is proportional to the square root of the voltage. The time factor becomes critical when one is dealing with sensitive ampholytes.

Thus, it would be desirable to develop techniques, and devices therefor, to allow for preparative separation of ampholytes with high resolution, at high speed, and with great reproducibility.

DETAILED DESCRIPTION OF THE INVENTION The present invention is concerned with new improved techniques for ampholyte separation and with devices therefor.

More specifically, the present invention relates to techniques for isoelectric focusing in pH gradients, and to valuable improvements in techniques allowing for rapid, large-scale, high resolution separation of ampholytes. Another aspect of the present invention is the development of a new series of devices which are amenable for use with the improved separation techniques.

The present techniques and instrumentation allow resolution of ampholytes to the theoretical limits of currently available carrier ampholytes. Compared to known instrumentation, the present devices allow an increase of approximately 10-fold in electric field strength per unit distance, and 30-fold in total applied electric potential; 3-fold in resolving power; 6-fold in power dissipation per unit volume, and up to 300-fold total power dissipation; 8-fold in speed; and 500-fold in weight of ampholyte material which can be separated. Thus, the present devices may be used at voltages up to 60 kv and can dissipate efficiently 6000 watts or more.

In one aspect of the present invention, there is an improved technique for isoelectric focusing of ampholytes which comprises conducting the focusing under conditions which allow for a large power input relative to the volume of the ampholyte solution, while keeping the temperature of the solution relatively constant.

In prior art instruments such as those of Valmet hereinabove mentioned, there is a significant increase in temperature of the ampholyte solution, even when utilizing a relatively low power input relative to the solution volume. Thus, in a typical instrument, there was reported to be approximately an average 7C rise in solution temperature under conditions of half a watt power input per milliliter of solution.

The present technique, and the instruments therefor, allow for a considerably higher power input, of at least 3 watts per milliliter of solution, resulting in an average 7C rise in solution temperature.

As mentioned above, it is critical to keep the temperature of the ampholyte solution relatively constant. Many of the ampholytes of biological importance are proteinaceous in nature and they are highly susceptible to denaturization by heat. As a rule, most isoeleetrie focusing of such materials is carried out at reduced temperatures, for example, about 0 to 5C, to avoid such denaturization or other harmful effects. A significant rise in temperature above this range can, in many cases, destroy the valuable biological activity or specificity of the molecules attempted to be purified.

In the prior art, as demonstrated by the devices of Valmet, such attempts to keep temperature increases to a minimum (which were not very effective) had to be accomplished by sacrificing the power input that could be used.

It is well known (see, for example, Svensson, Acta. Chemical. Scandinavia-a, 15, 425 (l96l)) that the resolving power of an isoelectric focusing system is directly related to the square root of the potential (voltage) applied to the system. While the resolving power is technically independent of the current, the time necessary for resolution (that is, to achieve an approximate equilibrium condition) is a function of the power input, that is, the wattage. The use of a low wattage instrument results in a lengthy period of time, e.g., two weeks, in order for isoelectric focusing to occur. Because of the instability of many of the ampholytes, such manipulation for an extended period of time, particularly if temperatures are not kept relatively low, is not desirable.

The present technique of utilizing a high wattage input without significant rise in solution temperature allows for isoelectric focusing with even higher resolution, and in much shorter periods of time, for example. about L5 hours, for an instrument of comparable volume.

In another aspect of the present invention, a highly in roved isoelectric focusing result may he received by sequential isoelectric focusing using a series of devices of successively smaller volume. without substantial dilution of the ampholyte solution.

In the prior art technique, as particularized by Valmet, the number of chambers in a particular isoelectric focusing unit was determinative of the resolution. Thus, the resolving power has been defined as the number of pH units in the pH gradient divided by half the number of chambers in the unit. For a pH gradient of 7 pH units and a unit with 30 chambers, the resolving power would be 0.47 pH units. To achieve a resolving power of 0.01 pH units with the same pH gradient (7 units), there would be required a cell with greater than l000 chambers.

By means of the present technique, resolutions as low as 0.01 pH units (theoretical limit of presently available carrier ampholytes) may be achieved, starting with a pH graddient of 7 pH units, by utilizing three successively smaller units having ten compartments each, for a total of 30 compartments. The key feature of the present method is the use of the series of successively smaller devices without substantial dilution of the ampholyte solution.

In a preferred method, each of the series of devices has the same number of chambers, and the ratio of the volume of each unit to the next smaller unit is approximately equal to the number of chambers in each unit.

Thus, for example, three typical units, having l chambers each, of volume 2100 ml, 2l5 ml and 25 ml, respectively, may be utilized. The crude ampholyte mixture is placed in the 2100 ml device with a carrier ampholyte establishing an artificial pH gradient of from pH 3 to pH (7 pH units), and subjected to isoelectric focusing. The one chamber (210 ml) containing the desired material is determined and its contents are trans ferred to the next smaller device (215 ml). Only minimal dilution is required to effect a quantitative ttransfer. The pH gradient in this device should be approximately 0.7 pH units. The isoelectric focusing is re peated and the chamber (approx. 22 ml volume) containing the desired material (pH gradient approx. 0.07 pH units) is again determined. After transfer to the next smallest device (25 ml) the focusing is repeated. After focusing, the chamber (approx. 2.5 ml) containing the desired material is determined and the product, now present in a pH gradient of approximately 0.01 pH units, is isolated by conventional techniques.

By utilizing the present methodology with devices allowing for increased power input as described above, each of the isoelectric focusing steps may be carried out in a relatively short period of time. Furthermore, the use of a total of chambers can produce a result which was heretofore only theoretically possible utilizing a device having at least 1,000 chambers.

Even larger devices, of about 20 liter volume, may be used to give a crude separation of large amounts of material, which may then be further purified on successively smaller units as described above.

The present method also offers a tremendous advantage over a dilution technique whereby the contents of a single chamber after isoelectric focusing are diluted and refocused in a device of the same size. Dilution of the ampholyte solution results in a loss of the buffering capacity of the carrier ampholytes so that the resolu tion attainable is diminished.

The process of improved isoclectric focusing by effecting a large power input while keeping a relatively constant temperature of the ampholyte solution is achieved by the use of a new series ofisoelectric focusing devices. A key feature of such devices is the efficient dissipation of heat generated by the large power input. by means of an improved cooling system. This efficient cooling system is made possible by careful selection of materials, by careful disposition of the cooling elements and by structural considerations allowing for stability in the presence of high voltages, simple and economical construction, and built-in safety features.

The improved electrophoresis devices for isoelectric focusing are characterized by being comprised of a sub stantially rectangular, box-shaped receptacle for receiving a solution of an ampholyte mixture to be separated, said receptacle having a bottom, two opposite end walls two opposite front and rear walls, two electrodes inserted in said receptacle proximate to said opposite end walls and adapted to be connected to a direct voltage source, said receptacle being provided with a plurality of impermeable walls, said impermeable walls extending parallel to said end walls and being disposed so that first alternate walls extend to the receptacle bottom and remaining alternate walls extend proximate to the receptacle bottom so as to divide the receptacle into a plurality of continously communicating U-tube chambers. The improvements provided by the present device comprise:

a. cooling means associated with said impermeable walls for causing highly efficient heat removal from the ampholyte solution which is proximate to said impermeable walls;

b. high voltage electrical insulation at all surfaces of the receptacle which contact the ampholyte solution during normal use; and

c. waterand high voltage-resistant sealing means for providing leak-proof seals at all internal joints of said receptacle which contact the ampholyte solution during normal use.

As provided above, one of the improvements in the present device is cooling means associated with the impermeable walls for causing highly efficient heat removal from the ampholyte solution which is proximate to said walls. In preferred embodiments, the cooling means includes means for passing coolant through channels in the impermeable walls extending from the front to the rear of the device.

There are three major components involved in the highly efficient heat removal. The first is the creation of turbulence at the boundary layer between coolant and the impermeable walls. The second factor is heat transfer (conduction) through the impermeable walls. The third factor is convection effects caused by a not perfectly uniform temperature of the ampholyte solution.

The boundary layer turbulence is caused by a shear effect of the coolant on the impermeable wall inside surfaces due to the coolant passing at high velocity through a narrow channel within the impermeable wall. The average velocity of coolant through the impermeable walls should be at least 1 ft/sec, and the width of the coolant channel should be between about l and 3 mm in order to cause the boundary layer turbulence.

Factors affecting the conduction and convection effects on the ampholyte solution include the thickness of the impermeable walls between the coolant and the ampholyte solution and the distance between adjacent impermeable walls. In order to create the desired effect, the thickness of the impermeable wall between the coolant and the ampholyte solution should not exceed 3 mm and is preferably between about 0.8 and 2.5 mm. The impermeable walls are pre-determinedly spaced, in relation to the receptacle size, so as to maximize con vention and conduction effects by providing high surface area contact with the ampholyte solution.

The distance between adjacent impermeable walls should not exceed l5 mm and is preferably between about I and mm.

Other factors having a more general effect on the rate of heat removal from the ampholyte solution are: nature of coolant, temperature of coolant, thermal conductivity of the impermeable walls, ampholyte solution, and coolant; viscosity of coolant and ampholyte solution; and so forth. One will normally control the temperature of the coolant, in conjunction with other parameters such as flow rate, in order to optimize heat dissipation, depending upon the nature and stability of the ampholyte being separated. A temperature range of from about 60to about +50C may be mentioned, with a preferred temperature range for most denaturable ampholytes of from about -l0 to about +10C.

A variety of conventional coolants may be utilized. Among these coolants are liquid coolants such as wa ter, mixtures of water with various organic solvents to allow for cooling without freezing below the normal freezing point of water, for example, ethylene glycol, isopropanol, and the like; flouorocarbon coolants; cooled gases such as air; and the like.

The coolant is allowed to travel through the channels in the impermeable walls, preferably by pumping means, adjusted so as to create the desired velocity as described above. In a suitable arrangement, coolant is introduced by appropriate means into a chamber, or plenum, either at the front or rear of the device, from which it is fed into the plurality of impermeable walls, exiting therefrom into another plenum from which the coolant is removed from the device. Preferably, a closed coolant system is utilized whereby expended coolant removed from the device is passed through external heat exchanging means to reduce the coolant temperature for recirculation back into the isoelectric focusing device.

In the following, the operation and advantages, as well as additional characteristic features of devices for isoelectric separation, according to the invention, will be further described with reference to the accompanying drawings which show, by way of example, a number of different embodiments of the invention.

In the drawings:

FIG. 1 shows schematically a first embodiment of a separation device according to the invention in a front cross-section;

FIG. 2 is a top view of the device in FIG. 1 taken along the line IIII in FIGv 1, with the top cover removed;

FIG. 3 shows schematically a second embodiment of a separation device according to the invention in front crosssection.

The isoelectric focusing device according to the invention illustrated in FIGS. I, 2 and 3, comprises a substantially rectangular, box-shaped receptacle 1 for receiving a solution of an ampholyte mixture to be separated. This receptacle has a bottom 2, two opposite end walls 3, 4 and two opposite front and rear walls 5, 6. Two electrodes 7, 8 are inserted in the receptacle proximate to the opposite end walls and are adapted to be connected to a direct voltage source. The device is provided with a plurality of impermeable walls 9, I0. These impermeable walls extend parallel to the end walls and are disposed so that first alternate walls 9 extend to the receptacle bottom, and remaining alternate walls 10 extend proximate to the receptacle bottom, preferably between about 1 and I0 mrn therefrom. The disposition of the impermeable walls results in the receptacle being divided into a plurality of continuously communicating U-tube chambers.

The number of U-tube chambers in any one device may vary from 3 to as high as I00, but preferred embodiments have approximately 10 U-tube chambers.

In preferred embodiments, the impermeable walls are essentially hollow, having a channel for the passage of coolant therethrough. In one embodiment of the present device, as illustrated in FIGS. I and 2, the impermeable walls are pictured as substantially rectangular in shape, having a rectangular channel for coolant. In FIG. 3, another embodiment is pictured having first impermeable walls 9 constructed of a series of vertically contiguous tubes for passage of coolant. In still another embodiment, all impermeable walls 9 and 10 are constructed of such vertically contiguous tubes.

Preferred embodiments of the present device further include additional cooling means II arranged above first alternate walls 9 so as to be in contact with the surface 12 of the ampholyte solution during normal use. In such a manner, effective cooling of ampholyte solu tion surface I2, at the point which would normally be minimally cooled by the other cooling means, is provided. Such cooling means II is normally constructed of similar material as impermeable walls 9 and 10. The shape of such means is not critical, although a substan tially circularly-shaped tube is preferred.

As shown in FIG. 2, coolant is introduced into the device through a plenum 13, passing from there through the plurality of impermeable walls 9, I0 and, if desired, through additional cooling means 11, and exiting through a plenum 14. Means 15 and 16, for introducing and removing coolant, communicating with plenums l3 and 14, are also provided. As mentioned above, various liquid and gaseous coolants may be use. Liquid fluorocarbons are particularly preferred for their property of being electrically nonconductive, so that the danger of an electrical short between the (aqueous) ampholyte solution and the cooling means caused by accidental breakage, leakage or otherwise, at the high operating voltages of the devices, would be significantly reduced.

Another feature of the present devices is that high voltage electrical insulation is provided at all surfaces of the receptacle which contact the ampholyte solution during normal use. This high voltage electrical insulation should have a dielectric strength of at least 500 volts/0.001 inch. A preferred insulation is glass. Since the devices operate at potentials above ground of up to 60,000 volts, the need for electrically non-conducting inner surfaces which will insulate against and be stable to the high voltages employed without shorting, is extremely critical and readily apparent.

If a material such as glass is used for insulation, it is normally of sufficient rigidity and strength to serve both as support for the structural members, as well as insulating means. If desired, suitable insulating materials may be used to coat otherwise non-insulating materials. For example, one may coat rigid materials such as metals with, for example, glass. For ease of construction, as well as economy, it is preferred that the same material be used both for support and insulation purposes, preferably a material such as glass.

A third feature of the present device is the use of water and high voltage-resistant sealing means for provid ing leak-proof seals at all internal joints of the receptacle which contact the ampholyte solution during normal use. The need for such leak-proof seals is readily apparent in view of the hazards attendant to the use of instruments involving such high voltages. lfa leak were to occur between the chamber containing ampholyte solution and, for example, aqueous cooling means, there would be created an extreme danger to the operator. The sealing means should be stable to the aqueous ampholyte solutions and be able to withstand, for extended time periods, the high voltages employed. Pre ferred sealing means include silicone-base sealers.

The receptacle 1 is preferably surrounded by an outer electrically non-conductive shield 17, and covered by an electrically non-conductive cover 18. The cover is preferably removable, allowing for sampling, etc., from the open Utube chambers during operation, a decided advantage over previous devices.

The devices of the present invention may be constructed in various sizes, for example, from about a volume (total volume of ampholyte solution) of about I ml to a volume of, for example, 50 or 100 liters. As explained above, a series of devices of decreasing volume may be used sequentially to purify ampholyte mixtures in preparative scale.

It is preferable to utilize the present instruments in conjunction with the above-described methodology, whereby improved ampholyte separations are achieved by performing the isoelectric fractionations at a power input of at least 3 watts per milliliter of ampholyte solution with a resultant average temperature increase of no more than 7C. When a series of such devices is additionally utilized in a sequential fashion, extremely rapid, high resolution separations of gram quantity samples of ampholyte mixtures heretofore unachievable in the art may be performed.

Among the types of ampholytes which may be separated utilizing the present devices and methodology, are proteins, particularly those of pharmaceutical inter est.

More specifically, any ampholyte which is soluble or recoverable at its isoelectric point without irreversible denaturation may be separated. Among these ampholytes are enzymes, hormones, cell particles, viruses, structural proteins, blood proteins, cerebrospinal fluid proteins, urine proteins, and the like.

As mentioned above, the technique of isoelectric focusing utilizes an artificially created pH gradient which is established by a mixture of synthetic compounds referred to as carrier ampholytes. These carrier ampholytes are generally of low molecular weight, and after the desired separation of the sample compounds is achieved, the desired materials are separated from the carrier ampholytes by standard chemical and physical techniques such as, for example, dialysis or chromatography.

The use of the present devices and techniques for the purification ofa typical ampholyte mixture is described below:

EXAMPLE I This example demonstrates the speed of separation of ampholyte mixtures utilizing the devices of the present invention:

The device used had a 25 ml capacity and contained 10 U-tube chambers. The receptacle itself was constructed entirely of glass with all joints being sealed with Dow-Corning No, 734 adhesive. The unit was constructed essentially as depicted in FIGS. 1 and 2. The distances between adjacent walls of the U-tubes, thickness of the glass walls between the coolant and the ampholyte solution, and the width of the cooling channels within the walls, were all 1 millimeter. The coolant utilized was a liquid fluorocarbon which entered the device at a temperature of 20C. The velocity of the coolant through each wall of the device was approximately two feet per second.

20 mg of whole blood was dissolved in 25 ml water containing 1% Ampholines (LKB), pH 35-10. The device was operated at a voltage of from 3,000 to 7,000 volts with an average power input of watts. After l .5 hours, of the hemoglobin (as determined by optical density measurement) was focused in one of the ten chambers of the device.

EXAMPLE 2 This example demonstrates the high resolution that can be obtained using successively smaller isoelectric focusing units in sequence without substantial dilution of the ampholyte mixture:

200 mg of the 100,000 X g supernatant of an aqueous extract of rat olfactory bulbs was subjected to isoelectric focusing This material was dialyzed and dissolved in 2 liters of 0.8% Ampholines (LKB), pH 3-6, and placed in a device substantially identical in design with that described in the previous example, except that additional cooling means 11 were not present. This device had a capacity of approximately 2 liters and contained 10 chambers. After reaching equilibrium on this device, the 200 ml Utube which contained most of the olfactory protein was then placed, after appropriate dilution with 20 ml of distilled water, in a 220 ml unit of identical design. After equilibrium was reached on this unit, the tube containing the maximum immunoreactive protein was then placed in a 25 ml unit, after appropriate dilution with 3 ml distilled water. After equilibrium was reached on this third instrument, the distribution of the desired protein was determined by immunoassay and discontinuous gel electrophoresis. The majority (90%) of the activity was concentrated into 5 tubes on this instrument in a final volume of ll ml, compared with the starting volume of 2000ml. This represents an approximately 50-fold purification. The olfactory protein had an approximately Gaussian distribution in the 5 tubes with a standard deviation of :t 0.0l0.0l5 pH units. The protein was then purified on DEAE column after removal of the Ampholines by dialysis.

I claim:

l. An improved method of preparative isoelectric focusing of the type utilizing passage of a direct current through an ampholyte solution, said ampholyte solution being comprised of a sample ampholyte mixture, a carrier ampholyte mixture and water, said carrier ampholyte mixture providing an artificial pH gradient, in a mulit-chamber isoclectric focusing device, wherein equal to the number of chambers in each device, in a manner such that the contents of one chamber of a device after isoelectric focusing are transferred to fill the next smaller device without substantial dilution of said ampholyte solution, and isoelectric focusing is repeated until the desired degree of sample ampholyte separation is achievedv 3. The method of claim 2 wherein the number of chambers in each device is about 10. 

1. AN IMPROVED METHOD OF PREPARATIVE ISOELECTRIC FOCUSING OF THE TYPE UTILIZING PASSAGE OF A DIRECT CURRENT THROUGH AN AMPHOLYTE SOLUTION, SAID AMPHOLYTE SOLUTION BEING COMPRISED OF A SAMPLE AMPHOLYTE MIXTURE, A CARRIER AMPHOLYTE MIXTURE AND WATER, SAID CARRIER AMPHOLYTE MIXTURE PROVIDING AN ARTIFICIAL PH GRADIENT, IN A MULIT-CHAMBER ISOELECTRIC FOCUSING DEVICE, WHEREIN THE IMPROVEMENT COMPRISES SEQUENTIALLY SUBJECTING SAID AMPHOLYTE MIXTURE, OR DESIRED PORTIONS THEREOF, TO ISOELECTRIC FOCUSING IN OF MULTI-CHAMBER DEVICES OF SUCCESSIVELY SMALLER VOLUME, WITHOUT SUBSTANTIAL DILUTION OF SAID AMPHOLYTE.
 2. The method of claim 1 wherein said sequential treatment utilizes a series of devices of successively smaller volume, such that each device has the same number of chambers, and the ratio of volume of each device to the next smaller device is approximately equal to the number of chambers in each device, in a manner such that the contents of one chamber of a device after isoelectric focusing are transferred to fill the next smaller device without substantial dilution of said ampholyte solution, and isoelectric focusing is repeated until the desired degree of sample ampholyte separation is achieved.
 3. The method of claim 2 wherein the number of chambers in each device is about
 10. 